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A spectrophotometer is a device which is used to determine the concentration of solid particles in any suspension especially a blood sample or transmittance of a solution.
A spectrophotometer is employed to measure the amount of light that a sample absorbs.
The instrument operates by passing a beam of light through a sample and measuring the intensity of light reaching a detector. The beam of light consists of a stream of photons.
When a photon encounters an analyte molecule (the analyte is the molecule being studied), there is a chance the analyte will absorb the photon. This absorption reduces the number of photons in the beam of light, thereby reducing the intensity of the light beam, which is then detected by the photo detector and given to display.
APPLICATIONS:
Spectrophotometer is used in various biology and biotechnology applications:
• to identify food dyes,
• perform protein assays of milk and protein drinks
• test for rates of photosynthesis
• use enzyme preparations to break down complex sugars
• measure the growth of bacterial culture